ANALGESIC, ANTI-INFLAMMATORY AND TOXICITY STUDIES ON METHANOLIC LEAF EXTRACT OF ROTHMANNIA LONGIFLORA SALISB (RUBIACEAE) IN RATS AND MICE
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
The herbalists in several African Countries have used Rothmannia longiflora decoctions as febrifugal and analgesic agent. The present study examined the analgesic, anti-inflammatory and toxicological profile of the methanolic extract of the plant in experimental animals. The preliminary phytochemical screening of the methanolic leaf extract of R.longiflora was carried out and revealed the presence of cardiac glycoside, steroids/terpenoids, tannins, saponins, flavonoids and carbohydrates. The acute toxic effect (median lethal dose values (LD50) of methanolic leaf extract of Rothmannia longiflora was carried out using Lork’s method in intraperitoneal and oral routes in mice and rats and were found to be above 5000mg/kg body weight. The anti-nociceptive effects of the extract was studied using acetic acid induced writhing test in mice, hot plate test in mice and formalin induced pain in rats. The anti-inflammatory effect was studied using Carrageenan induced paw oedema in rats. The extract at doses of 50-1000mg/kg significantly (P0.05) of the levels of Sodium (Na+ ), Potassium (K+ ), Chloride (Cl- ) ions, Urea and Creatinine compared to that of the control at the end of 30 days (sub-chronic) and 90 days (chronic) studies. In liver function test, there was no statistically significant difference (P>0.05) in the levels of AST, ALT, and ALP enzymes in all the treated groups compared to the control group after the 30 days studies. However, there was statistically significant difference with 500mg/kg and 1000mg/kg after the 90 days treatment. The Histopathological study showed no observable gross lesions during the periods of subchronic and chronic studies. vii These findings suggest that methanolic leaf extract of R.longiflora possesses analgesic and anti-inflammatory potentials/effects that justify the ethnomedical use of the plant in the treatment of painful and inflammatory conditions by the herbal practitioners. In addition, the results of toxicity studies showed that the extract is non-toxic to the kidneys and the heart during the sub-chronic and chronic toxicity studies however; mild fatty change was observed with 500mg/kg in the liver after the chronic studies.
Related Project Materials
EFFECT OF SMART CONTRACT ON FINANCIAL MARKETS
Background Of The Study
The emergence of advanced technologies has led to increased competition between...
THE IMPACT OF CONSTRUCTION INDUSTRY IN THE ECONOMIC DEVELOPMENT OF NIGERIA (A CASE STUDY OF IMO STATE)
ABSTRACT
This study was carried out to evaluate the impact of construction industry in the econom...
INVESTIGATION OF OCCUPATIONAL SAFETY AND HEALTH(OSH) COMPLIANCE LEVEL IN ADDRESSING COVID 19 PANDEMIC IN THE INDUSTRIAL SECTOR
BACKGROUND OF THE STUDY
In December 2019, the global population will be awoken to an outbreak of a disease known as Coro...
STRATEGIES FOR GENERATING REVENUE FOR PUBLIC LIBRARIES IN NIGERIA (A CASE STUDY OF IBOM E-LIBRARY, UYO)
ABSTRACT
A library is an organized collection of sources of information and similar resources, made accessible to a defi...
AN APPRAISAL OF INVESTOR CONFIDENCE IN THE NIGERIAN REAL ESTATE SECTOR
ABSTRACT
The overall view of the Nigerian investment environment can be deemed non-profitable to the plain eye. It is on...
IMPACT OF STRATEGIC MANAGEMENT ON PRODUCTIVITY AND PROFITABILITY OF A BUSINESS ORGANIZATION
Abstract
The study focused on strategic management as a tool for the attainment of organizational perfo...
IMPACT OF SALES PROMOTION ON CONSUMER BRAND LOYALTY
ABSTRACT:- This study is a research of the impact of sales promotion on consumer brand loyalty a study of co...
THE ROLE OF MORTGAGE INSTITUTION IN HOUSING IN NIGERIA (A CASE STUDY OF ANAMBRA HOME LIMITED (2006-2014)
ABSTRACT
This research work focuses on the “The role of mortgage institution in housing delivery in Nigeria. A cas...
RELATIONSHIP BETWEEN TEACHERS’ RECRUITMENT PROCEDURE AND STUDENTS’ ACHIEVEMENT IN COMMERCE IN FEDERAL GOVERNMENT GIRLS’ COLLEGES, IN NORTH WEST ZONE, NIGERIA
ABSTRACT
Relationship between teachers‟ recruitment procedure and students‟ achievement in commerce among Federal Government Girls‟ Colle...
A STATISTICAL ANALYSIS OF RATE OF INDUSTRIAL ACCIDENTS
ABSTRACT
This study was carried out on statistical ana...
